CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), FITC, eBioscience™

货号：11-0319-41,11-0319-42

规格：25 Tests,100 Tests

价格：851,1914

产品类型：流式抗体

品牌：eBioscience

抗原：CD31 (PECAM-1)

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：WM-59 (WM59)

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, FITC, eBioscience™
浓度：	5 µL/Test	用法：	5uL（0.5 µg）/test(Flow）

产品详细信息Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.Applications Reported: The WM-59 (WM59) antibody has been reported for use in flow cytometric analysis.Applications Tested: This WM-59 (WM59) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

数据

CD31 (PECAM-1) Antibody (11-0319-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC(Product # 11-4714-42) (blue histogram) or Anti-Human CD31 (PECAM-1) FITC (purple histogram). Cells in the granulocyte gate were used for analysis.

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参考文献：

1.Stem cell reportsTargeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.2.eLifeTGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells.3.Circulation researchEfficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9."11-0319 was used in Flow cytometry/Cell sorting to demonstrate that the CRISPR/Cas9 modification of epithelial cells is a powerful platform for vascular research and for regenerative medicine."AuthorsAbrahimi P,Chang WG,Kluger MS,Qyang Y,Tellides G,Saltzman WM,Pober JS4.OncotargetOxidized macrophage migration inhibitory factor is a potential new tissue marker and drug target in cancer.5.Nature protocolsDirect conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state."11-0319 was used in Immunofluorescence to develop a method to directly convert human mid-gestation lineage-committed amniotic fluid-derived cells into vascular endothelial cells."AuthorsGinsberg M,Schachterle W,Shido K,Rafii S

技术参数

产品应用 Flow；IF；IHC；MISC

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