CD25 Monoclonal Antibody (BC96), eFluor 450

货号：48-0259-41,48-0259-42

规格：25 Tests,100 Tests

价格：1151,2632

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：BC96

荧光染料：eFluor 450

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, eFluor 450, eBioscience™
免疫原性：		用法：	5uL（0.25µg）/test(Flow）

Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD25 Antibody (48-0259-41) in Flow Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) and of Mouse IgG1 kappa Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD25 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品：

细胞周期检测细胞凋亡检测细胞增殖活性检测

参考文献：

1. Scientific reportsCombinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Experimental and therapeutic medicineEquilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Journal of immunology (Baltimore, Md. : 1950)Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16976.htm