Rat IgG1 kappa Isotype Control, FITC

货号： 11-4301-81, 11-4301-82, 11-4301-85

规格：50 µg,100 µg,500 µg

价格：867,1526,2243

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBRG1

荧光染料：FITC

抗体类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and ELISA.Applications Tested: Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Scientific reportsHematopoietic not systemic impairment of Roquin expression accounts for intestinal inflammation in Roquin-deficient mice."11-4301 was used in Flow cytometry/Cell sorting to investigate the intestinal immunopathology caused by insufficient Roquin, showing that hematopoietic, not systematic, impairment of Roquin expression accounts for pathology."AuthorsMontufar-Solis D,Vigneswaran N,Nakra N,Schaefer JS,Klein JR 2.Journal of virologyProphylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice."11-4301 was used in Flow cytometry/Cell sorting to suggest that the RSV G protein not only contributes to disease, but also dampens the host immune response to infection."AuthorsBoyoglu-Barnum S,Chirkova T,Todd SO,Barnum TR,Gaston KA,Jorquera P,Haynes LM,Tripp RA,Moore ML,Anderson LJ 3.BloodCD81 is essential for the formation of membrane protrusions and regulates Rac1-activation in adhesion-dependent immune cell migration."11-4301 was used in Flow cytometry/Cell sorting to investigate the role of CD81 in dendritic cells, showing that it is essential for the formation of membrane protrusions and regulates Rac1-activation in adhesion-dependent migration."AuthorsQuast T,Eppler F,Semmling V,Schild C,Homsi Y,Levy S,Lang T,Kurts C,Kolanus W 4.European journal of haematologyc-Met signaling promotes IL-6-induced myeloma cell proliferation."11-4301 was used as a Control in experiments to indicate that HGF can potentiate the effects of IL-6 in myeloma proliferation and migration."AuthorsHov H,Tian E,Holien T,Holt RU,Våtsveen TK,Fagerli UM,Waage A,Børset M,Sundan A

技术参数

产品应用 Flow；ICC；IF；IHC

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