CD43 Monoclonal Antibody (eBioR2/60), FITC, eBioscience™

货号：11-0431-81,11-0431-82,11-0431-85

规格：50 µg,100 µg,500 µg

价格：957,1599,3198

产品类型：流式抗体

品牌：eBioscience

抗原：CD43

物种：小鼠

宿主：大鼠

抗体亚型：IgM

克隆号：eBioR2/60

荧光染料：FITC

抗体类型:	单抗	同型对照：	Rat IgM Isotype Control, FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.125 µg/test(Flow）

产品详细信息Description: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

数据

CD43 Antibody (11-0431-82) in FlowStaining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC(Product # 17-0452-82) and 0.06 µg of Rat IgM kappa Isotype Control FITC (Product # 11-4341-82)(left) or 0.06 µg of Anti-Mouse CD43 FITC (right). Cells in thelymphocyte gate were used for analysis.

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参考文献：

1.PloS oneAlkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity."11-0431 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics."AuthorsJonas BA,Johnson C,Gratzinger D,Majeti R2.eLifelncRNA requirements for mouse acute myeloid leukemia and normal differentiation."11-0431 was used in Flow cytometry/Cell sorting to assess lncRNA function via an in vivo RNAi screen in a model of acute myeloid leukemia."AuthorsDelás MJ,Sabin LR,Dolzhenko E,Knott SR,Munera Maravilla E,Jackson BT,Wild SA,Kovacevic T,Stork EM,Zhou M,Erard N,Lee E,Kelley DR,Roth M,Barbosa IA,Zuber J,Rinn JL,Smith AD,Hannon GJ3.Stem cell reportsDistinctive Mesenchymal-Parenchymal Cell Pairings Govern B Cell Differentiation in the Bone Marrow."11-0431 was used in Flow cytometry/Cell sorting to study the notion that bone marrow is a composite of specialised niches, providing distinctive functional units to regulate hematopoiesis."AuthorsYu VW,Lymperi S,Oki T,Jones A,Swiatek P,Vasic R,Ferraro F,Scadden DT4.Immunity and ageing : I and AThe immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.5.Clinical and developmental immunologyProlactin levels correlate with abnormal B cell maturation in MRL and MRL/lpr mouse models of systemic lupus erythematosus-like disease."11-0431 was used in Flow cytometry/Cell sorting to determine the level of PRL receptor expression during bone-marrow B-cell development and assess relevance in disease outcomes in lupus-prone murine models."AuthorsLegorreta-Haquet MV,Flores-Fernández R,Blanco-Favela F,Fuentes-Pananá EM,Chávez-Sánchez L,Hernández-González R,Tesoro-Cruz E,Arriaga-Pizano L,Chávez-Rueda AK

技术参数

产品应用 Flow

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