TER-119 Monoclonal Antibody (TER-119), APC-eFluor 780

货号：47-5921-82,47-5921-80

规格：100μg,25μg

价格：2075,0

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：TER-119

荧光染料：APC-eFluor 780

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationTER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TER-119 Antibody (47-5921-82) in Flow Staining of BALB/c bone marrow cells with Anti-Mouse CD45 FITC (Product # 11-0451-82) and 0.25 µg of Rat IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4031-82) (left) or 0.25 µg of Anti-Mouse TER-119 APC-eFluor® 780 (right).Total viable cells were used for analysis.

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参考文献：

1. Nature cell biologyDifferential cytokine contributions of perivascular haematopoietic stem cell niches. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Proceedings of the National Academy of Sciences of the United States of AmericaEngineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Circulation researchAMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis."47-5921 was used in Flow cytometry/Cell sorting to elucidate the role of the AMPKa2 catalytic subunit in vascular repair following ischemia."AuthorsAbdel Malik R,Zippel N,Frömel T,Heidler J,Zukunft S,Walzog B,Ansari N,Pampaloni F,Wingert S,Rieger MA,Wittig I,Fisslthaler B,Fleming I4. Nature communicationsPericyte-expressed Tie2 controls angiogenesis and vessel maturation."47-5921 was used in Flow cytometry/Cell sorting to expand and revise the current concepts on vascular Angiopoietin/Tie signalling and propose a bidirectional, reciprocal EC-pericyte model of Tie2 signalling."AuthorsTeichert M,Milde L,Holm A,Stanicek L,Gengenbacher N,Savant S,Ruckdeschel T,Hasanov Z,Srivastava K,Hu J,Hertel S,Bartol A,Schlereth K,Augustin HG5. The Journal of clinical investigationStromal cell cadherin-11 regulates adipose tissue inflammation and diabetes."47-5921 was used in Flow cytometry/Cell sorting to suggest that stromal fibroblasts expressing cadherin-11 regulate adipose tissue inflammation."AuthorsChang SK,Kohlgruber AC,Mizoguchi F,Michelet X,Wolf BJ,Wei K,Lee PY,Lynch L,Duquette D,Ceperuelo-Mallafré V,Banks AS,Brenner MB

技术参数

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