CD45R (B220) Monoclonal Antibody (RA3-6B2), FITC, eBioscience™

货号：11-0452-63,11-0452-81,11-0452-82,11-0452-85,11-0452-86

规格：10 µg ,50 µg ,100 µg ,500 µg ,1 mg

价格：0,529,831,1197,5245

产品类型：流式抗体

品牌：eBioscience

抗原：CD45R

物种：人/小鼠

宿主：大鼠

抗体亚型：其它

克隆号：RA3-6B2

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow）

产品详细信息Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

数据

CD45R (B220) Antibody (11-0452-63) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710(Product # 46-0032-82) and 0.25 µg of Rat IgG2a K Isotype Control FITC(Product # 11-4321-42) (left) or 0.25 µg of Anti-Human/Mouse CD45R (B220) FITC (right).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.JCI insightCeramide synthesis regulates T cell activity and GVHD development."11-0452 was used in Flow cytometry/Cell sorting to define the involvement of ceramides in the T-cell response to alloantigens present after allogeneic haematopoietic cell transplantation."AuthorsSofi MH,Heinrichs J,Dany M,Nguyen H,Dai M,Bastian D,Schutt S,Wu Y,Daenthanasanmak A,Gencer S,Zivkovic A,Szulc Z,Stark H,Liu C,Chang YJ,Ogretmen B,Yu XZ2.Scientific reportsAntibody-dependent fragmentation is a newly identified mechanism of cell killing in vivo."11-0452 was used in Flow cytometry/Cell sorting to investigate a novel antibody-dependent cell death pathway."AuthorsLiew PX,Kim JH,Lee WY,Kubes3.PLoS medicineIL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model."11-0452 was used in Flow cytometry/Cell sorting to suggest that IL33 may initiate early detrimental type 2 immune responses after trauma through ILC2 regulation of neutrophil IL5 production."AuthorsXu J,Guardado J,Hoffman R,Xu H,Namas R,Vodovotz Y,Xu L,Ramadan M,Brown J,Turnquist HR,Billiar TR4.PLoS pathogensBacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling."11-0452 was used in Flow cytometry/Cell sorting to study the effects of MAPK signalling pathways in group 3 innate immune lymphocytes using Bacillus anthracis."AuthorsSeshadri S,Allan DSJ,Carlyle JR,Zenewicz LA5.Journal of immunology (Baltimore, Md. : 1950)Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult Mice."11-0452 was used in Flow cytometry/Cell sorting to investigate the role of the Flt3 ligand in the development and regulation of innate lymphoid cells in fetal and adult mice."AuthorsBaerenwaldt A,von Burg N,Kreuzaler M,Sitte S,Horvath E,Peter A,Voehringer D,Rolink AG,Finke D

技术参数

产品应用 Flow；IF；IHC；MISC；ELISA；IHC (F)

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