Rat IgG1 kappa Isotype Control, PerCP-eFluor 710, eBioscience™

货号：46-4301-80

规格：25μg

价格：934

产品类型：大鼠同型对照

品牌：Thermo Fisher

物种：其它

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBRG1

荧光染料：PerCP-eFluor™ 710

抗体类型:	大鼠同型对照	同型对照：	IgG1, kappa
免疫原性：		用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: PerCP-eFluor® 710 Rat IgG1 Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: PerCP-eFluor® 710 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. Isotype control should be used at the same concentration as experimental antibody.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Journal of immunology (Baltimore, Md. : 1950)VprBP Is Required for Efficient Editing and Selection of Igκ+ B Cells, but Is Dispensable for Igλ+ and Marginal Zone B Cell Maturation and Selection."46-4301 was used as a Control in experiments to determine the contribution of the protein, VprBP, to B cell development and selection."AuthorsPalmer VL,Aziz-Seible R,Kassmeier MD,Rothermund M,Perry GA,Swanson PC2. Journal of neuroinflammationChronic thoracic spinal cord injury impairs CD8+ T-cell function by up-regulating programmed cell death-1 expression."46-4301 was used in Flow cytometry/Cell sorting to investigate CD8+ T-cell function by up-regulating programmed cell death-1 expression in chronic thoracic spinal cord injury."AuthorsZha J,Smith A,Andreansky S,Bracchi-Ricard V,Bethea JR

技术参数

产品应用 Ctrl;Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17002.htm