CD25 Monoclonal Antibody (OX39), PerCP-eFluor 710

货号：46-0390-80,46-0390-82

规格：25μg,100μg

价格：1132,2788

产品类型：流式抗体

品牌：Thermo Fisher

物种：大鼠

宿主：小鼠

抗体亚型：IgG

克隆号：OX39

荧光染料：PerCP-eFluor™ 710

抗体类型:	单抗	同型对照：	IgG1
免疫原性：		用法：	0.06 µg/test(Flow）

Description: The OX39 monoclonal antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineages as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.The OX39 clone has been shown to deplete CD25 cells in vivo.Applications Reported: This OX39 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX39 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD25 Antibody (46-0390-80) in Flow Staining of rat splenocytes with Anti-Rat CD4 FITC (Product # 11-0040-82) and 0.03 µg of Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or 0.03 µg of Anti-Rat CD25 PerCP-eFluor® 710 (right).Total viable cells were used for analysis.

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