CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), PerCP-eFluor 710

货号：46-0411-82,46-0411-80

规格：100μg,25μg

价格：3065,962

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：eBioMWReg30 (MWReg30)

荧光染料：PerCP-eFluor™ 710

抗体类型:	单抗	同型对照：	Rat IgG1 kappa Isotype Control, PerCP-eFluor 710, eBioscience™
免疫原性：		用法：	5uL（1 µg）/test(Flow）

Description: The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.Applications Reported: This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD41a Antibody (46-0411-82) in Flow Staining of mouse platelets with Anti-Mouse/Rat CD61 (Integrin beta 3) PE (Product # 12-0611-82) and 0.25 µg of Rat IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4301-80) (left) or 0.25 µg of Anti-Mouse CD41 PerCP-eFluor® 710 (right).Total viable events were used for analysis.

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参考文献：

1. Scientific reportsTargeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Molecular and cellular biologyThe Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Scientific reportsActivation of the TGFβ pathway impairs endothelial to haematopoietic transition.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Blood cancer journalNovel function of FAXDC2 in megakaryopoiesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5．Stem cell reportsMurine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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