CD3 Monoclonal Antibody (17A2), PerCP-eFluor 710

货号：46-0032-82,46-0032-80

规格：100μg,25μg

价格：3190,1132

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：17A2

荧光染料：PerCP-eFluor™ 710

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, PerCP-eFluor 710, eBioscience™
免疫原性：		用法：	0.25 µg/test(Flow）

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD3 Antibody (46-0032-82) in Flow Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.125 µg of Anti-Mouse CD3 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. International journal of molecular medicineSilybin attenuates LPS-induced lung injury in mice by inhibiting NF-κB signaling and NLRP3 activation. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Nature communicationsNK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Cellular and molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Journal of translational medicineA novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice."46-0032 was used in Flow cytometry/Cell sorting to study a novel multi-drug metronomic chemotherapy that significantly enhances the efficacy of intrinsic or vaccine-elicited tumor-specific cellular immunity."AuthorsTagliamonte M,Petrizzo A,Napolitano M,Luciano A,Rea D,Barbieri A,Arra C,Maiolino P,Tornesello M,Ciliberto G,Buonaguro FM,Buonaguro L5. Frontiers in immunologyDickkopf-3 Contributes to the Regulation of Anti-Tumor Immune Responses by Mesenchymal Stem Cells."46-0032 was used in Flow cytometry/Cell sorting to investigate whether DKK3 contributes to the immune suppression of anti-tumour responses by mesenchymal stem cells."AuthorsLu KH,Tounsi A,Shridhar N,Küblbeck G,Klevenz A,Prokosch S,Bald T,Tüting T,Arnold B

技术参数

产品应用 Flow

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