CD45.1 Monoclonal Antibody (A20), PerCP-Cyanine5.5

货号：45-0453-82,45-0453-80

规格：100μg,25μg

价格：3200,1164

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：小鼠

抗体亚型：IgG

克隆号：A20

荧光染料：PerCP-Cy5.5

抗体类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control, PerCP-Cyanine5.5, eBioscience™
免疫原性：		用法：	0.25 µg/test(Flow）

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.Applications Reported: This A20 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A20 antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD45.1 Antibody (45-0453-82) in Flow Staining of SJL splenocytes with 0.125 µg of Mouse IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4724-82) (open histogram) or 0.125 µg of Anti-Mouse CD45-1 PerCP-Cyanine5-5 (filled histogram). Total viable cells were used for analysis.

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参考文献：

1. The Journal of clinical investigationBiopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of experimental medicineEgr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Stem cell reportsMurine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. OncotargetMEK1 is required for the development of NRAS-driven leukemia."45-0453 was used in Flow cytometry/Cell sorting to consider the use of MEK1 as a tumour suppressor in the context of leukaemogenesis."AuthorsNowacka JD,Baumgartner C,Pelorosso C,Roth M,Zuber J,Baccarini M5. The Journal of clinical investigationTGF-β receptor maintains CD4 T helper cell identity during chronic viral infections."45-0453 was used in Flow cytometry/Cell sorting to uncover an eomesodermin-driven CD4 T cell program that is continuously suppressed by TGF-β signalling."AuthorsLewis GM,Wehrens EJ,Labarta-Bajo L,Streeck H,Zuniga EI

技术参数

产品应用 Flow

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