CD45R (B220) Monoclonal Antibody (RA3-6B2), PerCP-Cyanine5.5

货号：45-0452-80,45-0452-82

规格：25μg,100μg

价格：925,2534

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：RA3-6B2

荧光染料：PerCP-Cy5.5

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PerCP-Cyanine5.5, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the I{lpr/lprI} mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD45R (B220) Antibody (45-0452-82) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse CD3 eFluor® 450 (Product # 48-0032-82) and 0.25 µg of Rat IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (left) or 0.25 µg of Anti-Human/Mouse CD45R (B220) PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Nature cell biologyDifferential cytokine contributions of perivascular haematopoietic stem cell niches.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of experimental medicineEpithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Journal of immunology (Baltimore, Md. : 1950)Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization."45-0452 was used in Flow cytometry/Cell sorting to investigate how actin polymerization modulation restores lymphocyte migration in response to chemokines in HIV-1 infection."AuthorsCecchinato V,Bernasconi E,Speck RF,Proietti M,Sauermann U,D'Agostino G,Danelon G,Rezzonico Jost T,Grassi F,Raeli L,Schöni-Affolter F,Stahl-Hennig C,Uguccioni M4. Cell reportsLineage-Specific Genes Are Prominent DNA Damage Hotspots during Leukemic Transformation of B Cell Precursors."45-0452 was used in Flow cytometry/Cell sorting to demonstrate lineage-specific transcription predisposes genes in B cells to DNA damage."AuthorsBoulianne B,Robinson ME,May PC,Castellano L,Blighe K,Thomas J,Reid A,Müschen M,Apperley JF,Stebbing J,Feldhahn N5. PloS oneIntravaginal Chlamydia trachomatis Challenge Infection Elicits TH1 and TH17 Immune Responses in Mice That Promote Pathogen Clearance and Genital Tract Damage."45-0452 was used in Flow cytometry/Cell sorting to determine if intravaginal infection with occulogenital Chlamydia trachomatis is a possible mouse model for Chlamydia infection in humans."AuthorsVicetti Miguel RD,Quispe Calla NE,Pavelko SD,Cherpes TL

技术参数

产品应用 Flow

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