Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit,凋亡 流式
货号：A35136
规格：1 kit
价格：5505
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| This product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to violet-fluorescent Pacific Blue™ dye and dead cells using SYTOX™ AADvanced™ stain. After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation. There is very little spectral overlap between the two dyes, therefore very little compensation is needed. Each kit contains sufficient reagents for approximately 50 flow cytometry tests. |
| 数据： |
| Flow Cytometry dual parameter dot plot of Jurkat cells stained with Apoptosis Kit - Pacific Blue™ Annexin V/SYTOX® AADvanced™ *for flow cytometry* *50 tests*Jurkat cells (T-cell leukemia, human) treated with 10 µM camptothecin for four hours. Cells were treated with the reagents in Apoptosis Kit – Pacific Blue™ Annexin/SYTOX® AADvanced™ and analyzed by flow cytometry using 405 nm and 488 nm excitation. Note that the camptothecin-treated cells have a higher percentage of apoptotic cells (1B) than the basal level of apoptosis seen in the control cells (1A). A = apoptotic cells, V = viable cells, N = necrotic cells. |
| 相关产品： |
| 细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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