Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine7

货号：25-5981-81,25-5981-82

规格：50μg,100μg

价格：1897,3090

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：D7

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PE-Cyanine7, eBioscience™
免疫原性：		用法：	0.06µg/test(Flow）

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6A/E (Sca-1) Antibody (25-5981-81) in Flow Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.03 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.03 µg of Anti-Mouse Ly-6A/E (Sca-1) PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Nature communicationsInduced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. PloS oneMicrogrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. The Journal of clinical investigationFOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature medicineBone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. PloS oneZoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB."25-5981 was used in Flow cytometry/Cell sorting to study the effect of biphosphonates suppressed proteosteoclasts releasing PDGF-BB, and their role in coupling angiogenesis and osteogenesis in BRONJ manifestation."AuthorsGao SY,Zheng GS,Wang L,Liang YJ,Zhang SE,Lao XM,Li K,Liao GQ

技术参数

产品应用 Flow

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