CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), FITC

货号：11-0211-82

规格：100μg

价格：1649

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：其它

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)B)

产品详细信息Description: The monoclonal antibody eBio8D9 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which produce cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is some evidence which demonstrates their expression on macrophages. CD21 is a receptor for the complement component C3d and Epstein-Barr virus (EBV). In association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2-deficient mice display impaired inflammatory and humoral immune responses in vivo.The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other, suggesting that they bind to different epitopes.Applications Reported: This eBio8D9 (8D9) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio8D9 (8D9) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

数据

CD21/CD35 Antibody (11-0211-82) in FlowStaining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.125 µg of Rat IgG2a KIsotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse CD21/CD35 FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.The Journal of experimental medicineBAFF activation of the ERK5 MAP kinase pathway regulates B cell survival."11-0211 was used in Flow cytometry/Cell sorting to reveal a potentially critical role for the MEK5-ERK5 MAP kinase signalling pathway in BAFF-induced mature B cell survival and homeostatic maintenance of B2 cell numbers."AuthorsJacque E,Schweighoffer E,Tybulewicz VL,Ley SC2.Journal of immunology (Baltimore, Md. : 1950)Sox4 is required for the survival of pro-B cells."11-0211 was used in Flow cytometry/Cell sorting to investigate the role of Sox4 in development of mature B cells, showing that it is required for pro-B cell survival."AuthorsSun B,Mallampati S,Gong Y,Wang D,Lefebvre V,Sun X3.BloodDeletion of genes encoding PU.1 and Spi-B in B cells impairs differentiation and induces pre-B cell acute lymphoblastic leukemia."11-0211 was used in Flow cytometry/Cell sorting to investigate the impairment of differentiation of B cells and induction of pre-B cell acute lymphoblastic leukemia through the deletion of genes encoding PU.1 and Spi-1 in B cells."4.Journal of immunology (Baltimore, Md. : 1950)Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C."11-0211 was used in Flow cytometry/Cell sorting to demonstrate the role of the E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C in follicular B cell development."AuthorsDeKoter RP,Geadah M,Khoosal S,Xu LS,Thillainadesan G,Torchia J,Chin SS,Garrett-Sinha LA5.Nature immunologyHVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species."11-0211 was used in Flow cytometry/Cell sorting to investigate how HVCN1 modulates BCR signal strength through regulation of BCR-dependent generation of reactive oxygen species."AuthorsCapasso M,Bhamrah MK,Henley T,Boyd RS,Langlais C,Cain K,Dinsdale D,Pulford K,Khan M,Musset B,Cherny VV,Morgan D,Gascoyne RD,Vigorito E,DeCoursey TE,MacLennan IC,Dyer MJ

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