CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), PE-Cyanine7
货号：25-1522-82,25-1522-80
规格：100μg,25μg
价格：3313,1402
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：仓鼠
抗体亚型：IgG
克隆号：UC10-4B9
荧光染料：PE-Cy7
抗体类型: | 单抗 | 同型对照： | Armenian Hamster IgG Isotype Control, PE-Cyanine7, eBioscience™ |
免疫原性： | | 用法： | 0.5µg/test(Flow） |
Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly. Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Applications Reported: This UC10-4B9 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.Applications Tested: This UC10-4B9 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the FoxP3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4 degrees C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green LaserBackground/Target InformationCTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| CD152 (CTLA-4) Antibody (25-1522-82) in Flow Mouse splenocytes were unstimulated (left) or stimulated with Concanavalin A (Product # 00-4978) for 3 days (right). Cells were intracellularly stained with Anti-Mouse CD4 FITC (Product # 11-0041) and 0.25 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888) (blue histogram) or 0.25 µg of Anti-Mouse CD152 (CTLA-4) PE-Cyanine7 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Cells in the CD4+ gate were used for analysis. |
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