Phospho-DNA-PK (Ser2056) Polyclonal Antibody

货号：PA5-78130

规格：100 µL

价格：5593

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Carrier-protein conjugated synthetic peptide encom

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.68 mg/mL	用法：	1:100-1:1,000(ICC);1:100-1:1,000(IF)；1:500-1:3000(WB)

产品详细信息Positive Control: 293T, 293T (100 J/m2 UVC treatment)Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息This gene encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.

数据：

Phospho-DNA-PK (Ser2056) Antibody (PA5-78130) in IFImmunofluorescence analysis of DNA-PKCS was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-DNA-PK (Ser2056) Polyclonal Antibody (Product # PA5-78130) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear localization of DNA-PKCS. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Phospho-DNA-PK (Ser2056) Antibody (PA5-78130) in WBWestern blot analysis of Phospho-DNA-PKcs (Ser2056) in untreated (-) and treated (+) 293T cells using 60 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Phospho-DNA-PKcs (Ser2056) polyclonal antibody (Product # PA5-78130) using a dilution of 1:1000.

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