CD11b Monoclonal Antibody (M1/70), PE-Cyanine7

货号：25-0112-81,25-0112-82

规格：50μg,100μg

价格：1267,2019

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M1/70

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, PE-Cyanine7, eBioscience™
免疫原性：		用法：	0.125µg/test(Flow）

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

CD11b Antibody (25-0112-81) in FlowStaining of mouse bone marrow cells with 0.06 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD11b PE-Cyanine7 (purple histogram). Cells in the large scatter population were used for analysis.

数据

CD11b Antibody (25-0112-81) in Flow Staining of mouse bone marrow cells with 0.06 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD11b PE-Cyanine7 (purple histogram). Cells in the large scatter population were used for analysis.

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参考文献：

1. The Journal of clinical investigationA CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. JCI insightExperimental lupus is aggravated in mouse strains with impaired induction of neutrophil extracellular traps.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Mucosal immunologycorneal macrophages exhibit distinct characteristics and balance inflammatory responses after epithelial abrasion.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature medicineJNK1 negatively controls antifungal innate immunity by suppressing CD23 expression.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Nature communicationsRegulation of endothelial intracellular adenosine via adenosine kinase epigenetically modulates vascular inflammation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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