CD16/CD32 Monoclonal Antibody (93), PE-Cyanine7, eBioscience™

货号：25-0161-81

规格：50μg

价格：1237

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：PE-Cyanine7

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.25 µg/test(Flow）

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD16/CD32 Antibody (25-0161-81) in Flow Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Mouse CD16/CD32 PE-Cyanine7 (filled histogram). Total cells were used for analysis.

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参考文献：

1. Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. NatureMyeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. PloS oneSWEF Proteins Distinctly Control Maintenance and Differentiation of Hematopoietic Stem Cells."25-0161 was used in Flow cytometry/Cell sorting to investigate the role of SWAP-70 and DEF6 in hematopoietic stem and precursor cells."AuthorsRipich T,Chacón-Martínez CA,Fischer L,Pernis A,Kiessling N,Garbe AI,Jessberger R4. Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."25-0161 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone."AuthorsAmbrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ5. Journal for immunotherapy of cancerAgonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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