HLA-DR Monoclonal Antibody (LN3), APC

货号：17-9956-42,17-9956-41

规格：100 Tests,25 Tests

价格：2899,1132

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：LN3

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	5uL（0.0075µg）/test(Flow）

Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.Applications Reported: The LN3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationHLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

HLA-DR Antibody (17-9956-42) in Flow Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human HLA-DR APC (filled histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.

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参考文献：

1. Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Stem cell reportsLack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Circulation researchEfficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9."17-9956 was used in Flow cytometry/Cell sorting to demonstrate that the CRISPR/Cas9 modification of epithelial cells is a powerful platform for vascular research and for regenerative medicine."AuthorsAbrahimi P,Chang WG,Kluger MS,Qyang Y,Tellides G,Saltzman WM,Pober JS4. PLoS pathogensNovel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses."17-9956 was used in Flow cytometry/Cell sorting to provide a previously unknown mechanism for parasite-induced subversion of adaptive immunity."AuthorsPreston SG,Majtán J,Kouremenou C,Rysnik O,Burger LF,Cabezas Cruz A,Chiong Guzman M,Nunn MA,Paesen GC,Nuttall PA,Austyn JM5. Journal of inflammation researchAlleviation of insulin resistance and liver damage by oral administration of Imm124-E is mediated by increased Tregs and associated with increased serum GLP-1 and adiponectin: results of a phase I/II clinical trial in NASH."17-9956 was used in Flow cytometry/Cell sorting to examine the reported beneficial effects of Imm124-E for the treatment of organ damage."AuthorsMizrahi M,Shabat Y,Ben Ya'acov A,Lalazar G,Adar T,Wong V,Muller B,Rawlin G,Ilan Y

技术参数

产品应用 Flow

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