Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647

货号：A32733

规格：1mg

价格：3835

产品类型：二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor plus 647

36个不同种属、不同荧光的Alexa Fluor plus荧光二抗

点击表格中的货号，可跳转到相应的产品页。

	AF Plus Secondary Antibody products
	AF Plus 488	AF Plus 555	AF Plus 594	AF Plus 647	AF Plus 680	AF Plus 800
羊抗兔	A32731	A32732	A32740	A32733	A32734	A32735
羊抗鼠	A32723	A32727	A32742	A32728	A32729	A32730
羊抗鸡	A32931	A32932	A32759	A32933	A32934	A32935
驴抗羊	A32814	A32816	A32758	A32849	A32860	A32930
驴抗兔	A32790	A32794	A32754	A32795	A32802	A32808
驴抗鼠	A32766	A32773	A32744	A32787	A32788	A32789

产品优势：1. 更多荧光选择（从480到800），更多种属选择；2. 更好的光稳定性，更高信噪比，更高灵敏度，经过交叉吸附检测；3. 发布1年多已经有多达99篇高分文章引用；4. 适用于ICC/IF/WB等实验应用。

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10µg/mL(ICC);1-10µg/mL（IF);0.05-0.2µg/mL(WB)

To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.Background/Target InformationObtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments.Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32733) in IFImmunofluorescent analysis of postsynaptic density protein (PSD-95) in E18 Sparague Dawley primary cortical neuronal cells. The cells were fixed with 4% formaldehyde for 15 mins, permeabilized with 0.25% Triton X-100 in PBS for 10 mins, and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a PSD-95 rabbit polyclonal antibody (Product # 51-6900) at a dilution of 1:200 in 3% BSA in PBS for 1 hr at RT, and then incubated with Invitrogen Alexa Fluor Plus 647 goat anti-rabbit IgG secondary antibody (Product # A32733) at a dilution of 1:1000 for 1 hr at RT. Nuclei were stained with Hoechst 33342 (product # H3570). The image contains overlay of neuronal PSD-95 (far red) and nuclei (blue). Images were taken on a Zeiss LSM 710 confocal microscope at 40X magnification.

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参考文献：

1. Developmental cellCCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.AuthorsSmith MD,Harley ME,Kemp AJ,Wills J,Lee M,Arends M,von Kriegsheim A,Behrends C,Wilkinson S2. Journal of neuroinflammationRoles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype.AuthorsWu J,Sun L,Li H,Shen H,Zhai W,Yu Z,Chen G3. PLoS pathogensHypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter.AuthorsKraus RJ,Yu X,Cordes BA,Sathiamoorthi S,Iempridee T,Nawandar DM,Ma S,Romero-Masters JC,McChesney KG,Lin Z,Makielski KR,Lee DL,Lambert PF,Johannsen EC,Kenney SC,Mertz JE4. Nature communicationsActivity-dependent expression of Channelrhodopsin at neuronal synapses.AuthorsGobbo F,Marchetti L,Jacob A,Pinto B,Binini N,Pecoraro Bisogni F,Alia C,Luin S,Caleo M,Fellin T,Cancedda L,Cattaneo A5. Frontiers in physiologyAre Expressed in the Epithelium of Murine Airways and Other Non-gustatory Tissues.AuthorsLiu S,Lu S,Xu R,Atzberger A,Günther S,Wettschureck N,Offermanns S

技术参数

产品应用 ICC；IF；WB

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