CD150 Monoclonal Antibody (9D1), APC

货号：17-1501-63,17-1501-81,17-1971-82

规格：10μg,50μg,100μg

价格：0,1848,3163

产品类型：流式抗体

品牌：Thermo Fisher

物种：其它

宿主：大鼠

抗体亚型：IgG

克隆号：9D1

荧光染料：APC

抗体类型:	单抗	同型对照：	IgG1
免疫原性：		用法：	1 µg/test(Flow）

Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150 (cat. 12-1502).Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells.Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD150 Antibody (17-1501-81) in Flow Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.5 µg of Rat IgG1 kappa Isotype Control APC (Product # 17-4301-82) (left) or 0.5 µg of Anti-Mouse CD150 APC (right). Total viable cells were used for analysis

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参考文献：

1. Nature communicationsReprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors."17-1501 was used in Flow cytometry/Cell sorting to demonstrate how it is possible to reprogram mouse embryonic fibroblasts into engraftable blood progenitors."AuthorsCheng H,Ang HY,A El Farran C,Li P,Fang HT,Liu TM,Kong SL,Chin ML,Ling WY,Lim EK,Li H,Huber T,Loh KM,Loh YH,Lim B2. The Journal of experimental medicineWASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Molecular pharmacologyAryl hydrocarbon receptor activation in hematopoietic stem/progenitor cells alters cell function and pathway-specific gene modulation reflecting changes in cellular trafficking and migration."17-1501 was used in Flow cytometry/Cell sorting to support a molecular mechanism for an aryl hydrocarbon receptor ligand to disrupt the homeostatic cell signaling of HSCs."AuthorsCasado FL,Singh KP,Gasiewicz TA4. Journal of immunology (Baltimore, Md. : 1950)E47 controls the developmental integrity and cell cycle quiescence of multipotential hematopoietic progenitors."17-1501 was used in Flow cytometry/Cell sorting to show E47 controls the developmental integrity and cell cycle quiescence of hematopoietic progenitors."AuthorsYang Q,Kardava L,St Leger A,Martincic K,Varnum-Finney B,Bernstein ID,Milcarek C,Borghesi L

技术参数

产品应用 Flow

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