CD19 Monoclonal Antibody (HIB19), APC

货号：17-0199-41,17-0199-42

规格：25 Tests,100 Tests

价格：1215,2629

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：HIB19

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	5uL（0.125µg）/test(Flow）

Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD19 Antibody (17-0199-42) in Flow Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (open histogram) or Anti-Human CD19 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Developmental cellA Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to BAcute Lymphoblastic Leukemia-Associated ETV6-RUNX1....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Nature communicationsPeli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. ImmunityHuman Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature communicationsHSP70-Hrd1 axis precludes the oncorepressor potential of N-terminal misfolded Blimp-1s in lymphoma cells."17-0199 was used in Flow cytometry/Cell sorting to show that lymphoma-associated Blimp-1 mutants are selectively recognized by HSP70-Hrd1, which leads to their accelerated degradation."AuthorsWang WF,Yan L,Liu Z,Liu LX,Lin J,Liu ZY,Chen XP,Zhang W,Xu ZZ,Shi T,Li JM,Zhao YL,Meng G,Xia Y,Li JY,Zhu J5. Nature cell biologyMedial HOXA genes demarcate haematopoietic stem cell fate during human development.By clicking the link above, you will be redirected to the Benchsci website.Disclaime

技术参数

产品应用 Flow

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