CD25 Monoclonal Antibody (PC61.5), APC
货号：17-0251-81,17-0251-82
规格：50 μg,100μg
价格：1822,2840
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：PC61.5
荧光染料：APC
抗体类型: | 小鼠流式抗体 | 同型对照： | Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience |
浓度： | 0.2mg/mL | 用法： | 0.125 µg/test(Flow） |
Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| CD25 Antibody (17-0251-81) in FlowStaining of SJL splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.06 µg of Anti-Mouse CD25 APC. Total viable cells were used for analysis. |
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参考文献： |
| 1.The Journal of experimental medicineB cell depletion reduces the development of atherosclerosis in mice."17-0251 was used in Flow cytometry/Cell sorting to challenge the current paradigm that B cell activation plays an overall protective role in atherogenesis, and identify new antiatherogenic strategies based on B cell modulation."2.OncoimmunologyGrowth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression."17-0251 was used in Flow cytometry/Cell sorting to show that bone morphogenetic protein-4 causes immunosuppression by augmenting PD-L1 expression in the mesenchymal subset of lung cancer cells."3.OncoimmunologyLYG1 exerts antitumor function through promoting the activation, proliferation, and function of CD4+T cells."17-0251 was used in Flow cytometry/Cell sorting to reveal a tumor-inhibiting role for LYG1 through promoting the activation, proliferation, and function of CD4+T cells in antitumor immune responses, offering implications for novel tumor immunotherapy."4.Journal of immunology (Baltimore, Md. : 1950)Differential geminin requirement for proliferation of thymocytes and mature T cells."17-0251 was used in Flow cytometry/Cell sorting to gain insight into the in vivo role of Geminin in progenitor cell division and differentiation." |
QQ:1749072012