Rat IgG2b kappa Isotype Control, PE-Cyanine5
货号：15-4031-81,15-4031-82,15-4031-83
规格：50μg,100μg,200μg
价格：1649,2271,2580
产品类型：大鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：eB149/10H5
荧光染料：PE-Cy5
抗体类型: | 单抗 | 同型对照： | IgG2b, kappa |
免疫原性： | | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent (Flow) |
Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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参考文献： |
| 1.Journal of translational medicine Relationship of CD44+CD24-/low breast cancer stem cells and axillary lymph node metastasis. "15-4031 was used as a Control in experiments to investigate the proportion of CD44+CD24-/low breast cancer stem cells for the diagnosis of metastases in axillary lymph nodes, revealing that these stem cells may help clinicians improve diagnosis." AuthorsWei W,Hu H,Tan H,Chow LW,Yip AY,Loo WT |
QQ:1749072012
