IgM Monoclonal Antibody (II/41), PE-Cyanine5

货号：15-5790-81,15-5790-82

规格：50μg,100μg

价格：1855,3090

产品类型：流式抗体

品牌：Thermo Fisher

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：PE-Cy5

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PE-Cyanine5, eBioscience™
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow）

Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationIgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

IgM Antibody (15-5790-82) in Flow Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of RatIgG2a Isotype Control PE-Cyanine5 (Product # 15-4321-80) (left) or 0.06 µg of Anti-Mouse IgM PE-Cyanine5 (right). Total viable cells were used for analysis.

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参考文献：

1. Proceedings of the National Academy of Sciences of the United States of AmericaLigase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Genes and developmentConversion of T cells to B cells by inactivation of polycomb-mediated epigenetic suppression of the B-lineage program.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Nature communicationsEditing of mouse and human immunoglobulin genes by CRISPR-Cas9 system.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. PloS oneThe proximal J kappa germline-transcript promoter facilitates receptor editing through control of ordered recombination."15-5790 was used in Flow cytometry/Cell sorting to identify the proximal GT promoter as the first cis-element known to regulate the internal order of Jκ rearrangements."AuthorsVettermann C,Timblin GA,Lim V,Lai EC,Schlissel MS

技术参数

产品应用 Flow

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