TER-119 Monoclonal Antibody (TER-119), Biotin

货号：13-5921-81,13-5921-82,13-5921-85

规格：50μg,100μg,500μg

价格：822,1518,2534

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：TER-119

荧光染料：Biotin

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, Biotin, eBioscience™
免疫原性：		用法：	0.5µg/test(Flow）

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.Applications Reported: The TER-119 antibody has been reported for use in flow cytometric analysis.Applications Tested: The TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationTER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TER-119 Antibody (13-5921-81) in Flow Staining of BALB/c bone marrow cells with 0.25 µg of Rat IgG2b Isotype Control (Product # 12-4031-82) (open histogram) or 0.25 µg of Anti-Mouse TER-119 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the small scatter population were used for analysis.

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参考文献：

1. Development (Cambridge, England)Transient loss of venous integrity during developmental vascular remodeling leads to red blood cell extravasation and clearance by lymphatic vessels....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Biochemical and biophysical research communicationsPostnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Development (Cambridge, England)Cbx4 regulates the proliferation of thymic epithelial cells and thymus function.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. The Journal of clinical investigationLoss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms."13-5921 was used in Immunopurification to identify pleckstrin-2 as an effector of the JAK2/STAT5 pathway and a key factor in the pathogenesis of JAK2V617F-induced myeloproliferative neoplasms."AuthorsZhao B,Mei Y,Cao L,Zhang J,Sumagin R,Yang J,Gao J,Schipma MJ,Wang Y,Thorsheim C,Zhao L,Stalker T,Stein B,Wen QJ,Crispino JD,Abrams CS,Ji P5. eLifeAffinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells."13-5921 was used in Magnetic cell separation to investigate relationship between the strength of antigenic stimulation and the response of mouse CD4+ T cells."AuthorsAllison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

技术参数

产品类型 Flow

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