CD3e Monoclonal Antibody (145-2C11),功能抗体 Functional Grade, eBioscience™

货号：16-0031-81,16-0031-82,16-0031-85,16-0031-86

规格：50 μg,100 μg,500 μg,1 mg

价格：967,1413,2720,4947

产品类型：流式抗体

品牌：eBioscience

抗原：CD3

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：145-2C11

荧光染料：其它

小鼠T细胞激活扩增protocol点击下方“实验方法PDF下载”

抗体类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control, Functional Grade, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow)

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the Best Protocols webpage to view a protocol for in vitro T-cell activation.

Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Background/Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3e Antibody (16-0031-81) in FlowStaining of mouse splenocytes with Anti-Mouse CD3e FITC (left) or PE (right).Appropriate isotype controls were used (open histogram). Total viable cells wereused for analysis.

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参考文献：

1.Nature communicationsThe ER membrane-anchored ubiquitin ligase Hrd1 is a positive regulator of T-cell immunity.2.PloS oneT cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.3.Nature communicationsIntratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.4.EBioMedicineDendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.5.The Journal of investigative dermatologyAuf1/Hnrnpd-deficient mice develop pruritic inflammatory skin disease."16-0031 was used in Immunohistochemistry to highlight HNRNPD and mRNA stability as important for the processes of leukocyte recruitment and inflammatory activation within the skin."AuthorsSadri N,Schneider RJ

技术参数

产品应用 Flow；IF；IHC；MISC；IHC (Free)；IHC (P)；WB；IHC (F)；CHIP；ELISA；TCA

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