Rat IgM Isotype Control, APC
货号:17-4341-82,17-4341-81
规格:100 μg,50μg
价格:2341,0
产品类型:大鼠同型对照
品牌:eBioscience
物种:其它
宿主:大鼠
抗体亚型:IgM
荧光染料:APC
Product Details
TESTED APPLICATIONS | DILUTION |
---|
Control (Ctrl) | Assay-Dependent |
Flow Cytometry (Flow) | Assay-Dependent |
Tested Species reactivity | Not Applicable |
Host / Isotype | Rat / IgM, kappa |
Class | Control |
Type | Isotype Control |
Clone | eBRM |
Conjugate | APC |
Form | Liquid |
Concentration | 0.2 mg/mL |
Purification | Affinity chromatography |
Storage buffer | PBS, pH 7.2, with 0.1% gelatin |
Contains | 0.09% sodium azide |
Storage conditions | 4° C, store in dark, DO NOT FREEZE! |
RRID | AB_470183 |
Product Specific Information
Description: The monoclonal rat IgM, kappa is useful as an isotype control immunoglobulin.Applications Reported: Rat IgM Isotype Control APC has been reported for use in flow cytometric analysis.Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
Background/Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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