PE Mouse Anti-Human CD16b Clone CLB-gran11.5 (RUO)

货号：550868

规格：100 Tests

价格：3858

产品类型：流式抗体

品牌：BD

抗原：CD16b

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：CLB-gran11.5

荧光染料：PE

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	20 uL/test(Flow）

Reacts with CD16b, a glycosyl phosphatidyl inositol-anchored (GPI) protein expressed on human neutrophils. Human CD16 is the low affinity Fc gamma receptor III (Fc gamma RIII) and shows two distinct forms coded by two linked genes. One form is CD16a, a polypeptide-anchored form (Fc gamma RIIIA), present on natural killer cells and macrophages. The other form is CD16b the GPI-anchored form, Fc gamma RIIIB, found on human neutrophils. CD16b is polymorphic and the two codominant alleles are referred to as Neutrophil Antigen 1 (NA1) and Neutrophil Antigen 2 (NA2). Clone CLBgran11.5 reacts with neutrophils expressing the NA1 molecule. CD16b has been reported to participate in immune complex binding by resting neutrophils and also in neutrophil transendothelial migration by interacting with integrins during the inflammation response process.FORMAT

• Format

• PE

• Excitation Source

• Blue 488 nm,Green 532 nm,Yellow/Green 561 nm

• Excitation Max

• 496nm

• Emission Max

• 578nm

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.PRODUCT NOTICESThis reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.Source of all serum proteins is from USDA inspected abattoirs located in the United States.

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参考文献：

Nagarajan S, Anderson M, Ahmed SN, Sell KW, Selvaraj P. Purification and optimization of functional reconstitution on the surface of leukemic cell lines of GPI-anchored Fc gamma receptor III. J Immunol Methods. 1995; 184(2):241-251.View reference Nagarajan S, Chesla S, Cobern L, Anderson P, Zhu C, Selvaraj P. Ligand binding and phagocytosis by CD16 (Fc gamma receptor III) isoforms. Phagocytic signaling by associated zeta and gamma subunits in Chinese hamster ovary cells. J Biol Chem. 1995; 270(43):25762-25770.View reference Nagarajan S, Venkiteswaran K, Anderson M, Sayed U, Zhu C, Selvaraj P. Cell-specific, activation-dependent regulation of neutrophil CD32A ligand-binding function. Blood. 2000; 95(3):1069-1077.View reference Sendo F, Suzuki K, Watanabe T, Takeda Y, Araki Y. Modulation of leukocyte transendothelial migration by integrin-associated glycosyl phosphatidyl inositol (GPI)-anchored proteins. Inflamm Res. 1998; 47(3):S133-S136.View reference

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