CD59 (Protectin) Monoclonal Antibody (OV9A2), FITC

货号：11-0596-41,11-0596-42

规格：25 Tests,100 Tests

价格：0,2055

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：OV9A2

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 μL (0.125 μg)/test(Flow)

产品详细信息Description: This OV9A2 monoclonal antibody reacts with human CD59, which is also known as Protectin. This GPI-linked membrane glycoprotein shares structural homology with the murine Ly-6 superfamily. CD59 is expressed on all human lymphocytes, monocytes, granulocytes, and erythrocytes. This protein is also expressed on non-hematopoietic cells, including endothelial cells and neurons. By binding the complement components C8 and C9, CD59 inhibits assembly of the membrane attack complex and cytolytic activity by complement. CD59 interacts with CD2 to modulate T cell adhesion, and also plays a role in T cell activation. Finally, altered expression of CD55 and CD59 on human peripheral blood cells has been observed in patients with systemic lupus erythematosus (SLE).Crossblocking studies indicate that the OV9A2 monoclonal antibody binds the same epitope as MEM-43.Applications Reported: This OV9A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OV9A2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). CD59 is the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 also associates with C5b-8 complex and counteracts appropriate formation of cytolytic pore within the plasma membrane. Further, CD59 is a low-affinity ligand of human CD2, causes T cell costimulation, and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. Mutations in this gene cause CD59 deficiency, a disease involving hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants of CD59 have been identified.

数据

CD59 (Protectin) Antibody (11-0596-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD59 (Protectin) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Experimental and therapeutic medicineMALP-2, an agonist of TLR6, promotes the immune status without affecting the differentiation capacity of umbilical cord mesenchymal stem cells."11-0596 was used in Flow cytometry/Cell sorting to assess the role of TLR6 in the immune status of umbilical cord mesenchymal stem cells, by stimulation with the TLR6 agonist MALP-2."AuthorsWu X,Xu L,Shen Y,Yu N,Zhang Y,Guo T2.PloS oneIncreased expression of complement regulators CD55 and CD59 on peripheral blood cells in patients with EAHEC O104:H4 infection."Published figure using CD59 (Protectin) monoclonal antibody (Product # 11-0596-42) in Flow Cytometry"

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