TGF beta-1 Human/Mouse Uncoated ELISA Kit with Plates/人/小鼠TGF beta-1 ELISA试剂盒含未包被板
货号：88-8350-22,88-8350-76,88-8350-86
规格：2 x 96 tests,20 x 96 tests,10 x 96 tests
价格：2856,9393,6537
产品类型：自定义ELISA
品牌：Thermo Fisher
物种：人/小鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 8 pg/mL |
| 检测范围 | 8-1,000 pg/mL |
样本类型/体积 | 血浆 20 μL 血清 20 μL 上清液 100 μL |
操作时间 | 1 hr 45 min |
测试时间 | 24 hr 30 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2 X 96 Tests |
组份 | - 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 - 包被缓冲液：10X PBS ELISA包被缓冲液 - 检测稀释液：5X浓缩液 - 检测酶：Pre-titrated Avidin-HRP - 底物：1X TMB溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar flat-bottom plates（含产品目录号，后缀 -22，-76，-86） |
储存温度 | 2-8℃ |
| Protein name | TGF beta-1 |
| Protein aliases | LAP, Latency-associated peptide, prepro-transforming growth factor beta-1, regulatory protein, TGF-beta 1, TGF-beta-1, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein, transforming growth factor-beta 1 |
物种（Tested） | 小鼠，人呢 |
试剂盒类型 | Uncoated ELISA Kit with Plates |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene ID | (Human) 7040,(Mouse) 21803 |
| Gene aliases | CED, DPD1, LAP, TGF-beta1, TGFB, Tgfb-1, TGFB1, TGFbeta, TGFbeta1 |
| Gene symbol | TGFB1, Tgfb1 |
UniProt ID | (Human) P01137,(Mouse) P04202 |
| The Human/Mouse Transforming Growth Factor beta 1 (TGFβ1) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human/mouse TGFβ1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人/小鼠转化生长因子β1（TGFβ1）未包被ELISA试剂盒包含预先匹配的抗体对，板和试剂，用于进行定量酶联免疫吸附测定（ELISA），以检测和定量人/小鼠TGFβ1的蛋白质水平。 需要洗涤缓冲液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被到微孔板孔的底部，这是一整夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.TGF beta-1（TGFB1，转化生长因子Beta 1）是细胞因子转化生长因子β超家族的多肽成员，几乎在组织中普遍存在。转化生长因子（TGF）-b作为潜在复合物以其前结构域形式存储在细胞外基质中。 TGFβ-1的激活需要aV整联蛋白与前结构域中的RGD序列结合，并在该结构域上施加作用力，该作用域由潜在的TGF-b结合蛋白保持在细胞外基质中。潜在形式是TGFβ-1，TGF-β前体的氨基末端部分，称为TGF-LAP（TGF-潜伏期相关肽）和特定结合蛋白（称为LTBP）的复合物。 TGF beta-1有助于调节许多细胞类型的增殖，分化，粘附，迁移。许多细胞具有TGFβ受体，并且该蛋白可以正负调节许多其他生长因子。 TGFβ-1裂解为潜伏期相关肽和成熟的TGFβ-1肽，并以由TGFB1同型二聚体，LAP同型二聚体和潜在TGFB1结合蛋白组成的潜伏形式存在，或在活性形式由TGFβ-1同型二聚体组成。成熟的肽还可以与其他TGFβ家族成员形成异二聚体。 TGFβ-1的基因在肿瘤细胞中经常被上调，该基因的突变导致Camurati-Engelmann病和囊性纤维化。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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