Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 532/羊抗兔 IgG (H+L)交叉吸附荧光二抗， Alexa Fluor 532

货号：A-11009

规格：500 µL

价格：3223

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 532

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	4 µg/mL(ICC);4 µg/mL（IF)

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11009) in IFImmunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 532 was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 532 (Product # A-11009) was used at a concentration of 4µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1.PLoS pathogensAutophagy Is an Innate Mechanism Associated with Leprosy Polarization."A11009 was used in immunocytochemistry to investigate the impact of autophagy on Mycobacterium leprae infection"AuthorsSilva BJ,Barbosa MG,Andrade PR,Ferreira H,Nery JA,Côrte-Real S,da Silva GM,Rosa PS,Fabri M,Sarno EN,Pinheiro RO2.The Journal of biological chemistryMolecular Determinants of Kv1.3 Potassium Channels-induced Proliferation."A11009 was used in immunocytochemistry to find the Kv1.3 domains involved in a proliferation-associated signaling mechanism"AuthorsJiménez-Pérez L,Cidad P,Álvarez-Miguel I,Santos-Hipólito A,Torres-Merino R,Alonso E,de la Fuente MÁ,López-López JR,Pérez-García MT3.CellLigand-Dependent Modulation of G Protein Conformation Alters Drug Efficacy."A11009 was used in western blot to explore the molecular basis by which G proteins regulate efficacy"AuthorsFurness SGB,Liang YL,Nowell CJ,Halls ML,Wookey PJ,Dal Maso E,Inoue A,Christopoulos A,Wootten D,Sexton PM4.Biomedical optics expressMultimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence."A11009 was used in immunohistochemistry to combine optical coherence tomography with fluorescence lifetime imaging microscopy to study human and rabbit tissues"AuthorsShrestha S,Serafino MJ,Rico-Jimenez J,Park J,Chen X,Zhaorigetu S,Walton BL,Jo JA,Applegate BE5.Science (New York, N.Y.)Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging.AuthorsSzymborska A,de Marco A,Daigle N,Cordes VC,Briggs JA,Ellenberg J

技术参数

产品应用 ICC；IF；MISC；WB

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