Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 532/羊抗鼠IgG (H+L)交叉吸附荧光二抗,Alexa Fluor 532

货号：A-11002

规格：1 mg

价格：3223

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 532

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	2 µg/mL(ICC);2 µg/mL（IF);1-10 µg/mL(Flow)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11002) in IFImmunofluorescence analysis of Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 532 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 532 (Product # A-11002) was used at a concentration of 2 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11002) in IFA= 10 µg/mL Primary Antibody, No BRDU incubation 2nd Antibody: A11002 1:200 dilution (DAPI=Blue), B= No Primary antibody, Hela cells incubated in 30 µg/mL for 24hrs, 2nd Antibody: Product # A11002 1:200 dilution (DAPI= Blue), C= 2.5 µg/mL Primary Antibody, Hela cells incubated in 30 µg/mL for 24hrs2nd Antibody: Product # A11002 (Only Blue/Dapi shown), D= 2.5 µg/mL Primary Antibody, Hela cells incubated in 30 µg/mL for 24hrs 2nd Antibody: Product # A11002 (Only red/Alexa 532 shown), D= 2.5 µg/mL Primary Antibody, Hela cells incubated in 30 µg/mL for 24hrs 2nd Antibody: Product # A11002 (Only red/Alexa 532 shown). Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11002) in IFA= 10 µg/mL Primary Antibody, No BRDU incubation 2nd Antibody: Product # A11002 1:200 dilution (DAPI=Blue), B= No Primary antibody, Hela cells incubated in 30 µg/mL for 24hrs, 2nd Antibody: Product # A11002 1:200 dilution (DAPI= Blue), C= 2.5 µg/mL Primary Antibody, Hela cells incubated in 30 µg/mL for 24hrs 2nd Antibody: Product # A11002 (Only Blue/Dapi shown), D= 10 µg/mL Primary Antibody, Hela cells incubated in 30 µg/mL for 24hrs 2nd Antibody: Product # A11002 (Only red/Alexa 532 shown). All cells were mounted using Prolong Antifade Mounting Medium (P7481). Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11002) in IFA bovine pulmonary artery endothelial cell labeled with mouse monoclonal anti-a-tubulin antibody (Product # A11126) in combination with Alexa Fluor 532 goat anti-mouse IgG antibody (Product # A-11002) to stain microtubules. The image was acquired using a bandpass filter set (excitation/emission 535 +- 17.5/590 +- 17.5 nm).

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参考文献：

1.Nature communicationsIFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMP.2.PLoS neglected tropical diseasesExpression of CD64 on Circulating Neutrophils Favoring Systemic Inflammatory Status in Erythema Nodosum Leprosum.3.The Journal of biological chemistryMolecular Determinants of Kv1.3 Potassium Channels-induced Proliferation.4.Biomedical optics expressSimple buffers for 3D STORM microscopy.5.Science (New York, N.Y.)Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging.

技术参数

产品应用 Flow；ICC；IF；IHC；MISC

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