Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 790/羊抗兔IgG (H+L)交叉吸附，Alexa Fluor 790

货号：A11369

规格：500μl

价格：3517

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 790

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抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（Flow）；1-10 μg/mL (ICC)；1-10 μg/mL (IF)； 1：10000 (WB)

This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A11369) in WBGoat anti-Rabbit IgG (H+L) Alexa Fluor 790 Secondary Antibody in WB Western blot analysis of total Cadherin and N-Cadherin was performed by loading 2 µL SeeBlue® Plus2 Prestained Protein Ladder (Product # LC5925), 50 µg of MDCK cell lysates and 10 µg mouse heart lysate per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Total cadherin was detected using a rabbit antibody (Product # 71-7100) and N-Cadherin was detected using a mouse antibody (Product # 33-3900), both at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform. The blot was then incubated with goat anti-rabbit IgG-Alexa Fluor 790 secondary antibody (Product # A11369) and goat anti-mouse IgG-Alexa Fluor 680 secondary antibody (Product # A-21058) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Images generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center.

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参考文献：

1. PloS oneDistinct repertoires of microRNAs present in mouse astrocytes compared to astrocyte-secreted exosomes."A11369 was used in western blot to describe the presence miRNAs contained within exosomes secreted by astrocytes"AuthorsJovičić A,Gitler AD2. Molecular biology of the cellComparative assessment of fluorescent proteins for in vivo imaging in an animal model system."A11369 was used in western blot to quantitatively assess fluorescent protein properties in vivo using Caenorhabditis elegans"AuthorsHeppert JK,Dickinson DJ,Pani AM,Higgins CD,Steward A,Ahringer J,Kuhn JR,Goldstein B3. Human molecular geneticsThe transcription coactivator ASC-1 is a regulator of skeletal myogenesis, and its deficiency causes a novel form of congenital muscle disease."A11369 was used in western blot to identify ASC-1 as a regulator of late myogenic differentiation and propose that myotube growth defects are a novel myopathic mechanism"AuthorsDavignon L,Chauveau C,Julien C,Dill C,Duband-Goulet I,Cabet E,Buendia B,Lilienbaum A,Rendu J,Minot MC,Guichet A,Allamand V,Vadrot N,Fauré J,Odent S,Lazaro L,Leroy JP,Marcorelles P,Dubourg O,Ferreiro A4. Proceedings of the National Academy of Sciences of the United States of AmericaLoss of O-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration."A11369 was used in western blot to characterize mice with a forebrain-specific loss of O-GlcNAc glycosylation"AuthorsWang AC,Jensen EH,Rexach JE,Vinters HV,Hsieh-Wilson LC

技术参数

产品应用 ICC;IF;WB;Flow

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