TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), Biotin, eBioscience™

货号：13-8863-82,13-8863-80

规格：100 µg,25 µg

价格：1442,822

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgM

荧光染料：Biotin

类型:	单抗	同型对照：	IgM
免疫原性：		用法：	0.25 µg/test(Flow)

Product Specific InformationDescription: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embryonal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.Target InformationTRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-60 (Podocalyxin) Antibody (13-8863-82) in FlowStaining of 2102Ep human embryonal carcinoma cell line with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Human TRA-1-60 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

1.Journal of virologyHigh-risk human papillomavirus E6 protein promotes reprogramming of Fanconi anemia patient cells through repression of p53 but does not allow for sustained growth of induced pluripotent stem cells."13-8863 was used in Immunofluorescence to utilize the oncogenes of high-risk human papillomavirus 16 (HPV16) to overcome the resistance of FA patient cells to reprogramming."AuthorsChlon TM,Hoskins EE,Mayhew CN,Wikenheiser-Brokamp KA,Davies SM,Mehta P,Myers KC,Wells JM,Wells SI2.PloS oneDerivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations.3.Journal of virologyHigh-risk human papillomavirus E6 protein promotes reprogramming of Fanconi anemia patient cells through repression of p53 but does not allow for sustained growth of induced pluripotent stem cells."13-8863 was used in Immunofluorescence to utilize the oncogenes of high-risk human papillomavirus 16 (HPV16) to overcome the resistance of FA patient cells to reprogramming."AuthorsChlon TM,Hoskins EE,Mayhew CN,Wikenheiser-Brokamp KA,Davies SM,Mehta P,Myers KC,Wells JM,Wells SI4.Stem cell reviewsComparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).

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