Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 647/羊抗兔 IgG (H+L) Superclonal™二抗，Alexa Fluor 647
货号：A27040
规格：1mg
价格：3337
产品类型：荧光二抗
品牌：Thermo Fisher
物种：兔
宿主：山羊
抗体亚型：IgG
荧光染料：Alexa Fluor 647
点击查看所有Alexa Fluor荧光二抗
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Recombinant full-length protein | 用法： | 2 μg/mL(ICC);2 μg/mL（IF) |
The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig and donkey IgG is observed.Background/Target InformationSuperclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.
数据 |
| Rabbit IgG (H+L) Secondary Antibody (A27040) in IFImmunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody Alexa Fluor® 647 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA516891) The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody Alexa Fluor® 647 conjugate (Product # A27040) was used at a concentration of 2 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
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参考文献： |
| 1.Nature methods An E3-ligase-based method for ablating inhibitory synapses. "A27040 was used in immunocytochemistry to develop a simple, reversible method for modulating inhibitory synaptic input onto genetically determined cells" AuthorsGross GG,Straub C,Perez-Sanchez J,Dempsey WP,Junge JA,Roberts RW,Trinh le A,Fraser SE,De Koninck Y,De Koninck P,Sabatini BL,Arnold DB |
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