Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647/鸡抗大鼠 IgG (H+L) 交叉吸附，Alexa Fluor 647

货号：A-21472

规格：500μl

价格：3563

产品类型：荧光二抗

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：IgG

荧光染料：Alexa Fluor 647

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（Flow）；1-10 μg/mL (ICC)；1-10 μg/mL (IF)

Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors. Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.Background/Target InformationAnti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21472) in IFImmunofluorescence analysis of Chicken anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 647 was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL Rat primary antibody for 3 hours at room temperature. Chicken anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 647 (Product # A-21472) was used at a concentration of 2µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Brain structure and functionCellular architecture and transmitter phenotypes of neurons of the mouse median raphe region."A21472 was used in immunohistochemistry to determine the neuron composition of the median raphe region"AuthorsSos KE,Mayer MI,Cserép C,Takács FS,Szőnyi A,Freund TF,Nyiri G2. Molecular medicine reportsEffect of exercise-induced neurogenesis on cognitive function deficit in a rat model of vascular dementia."A21472 was used in immunohistochemistry to ask if hippocampal neurogenesis induced by exercise improves cognitive deficit in a rat model of vascular dementia"AuthorsChoi DH,Lee KH,Lee J3. The Journal of clinical investigationHepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment."A-21472 was used in immunohistochemistry - paraffin section to elucidate the origin of hepatocellular carcinoma."AuthorsMu X,Español-Suñer R,Mederacke I,Affò S,Manco R,Sempoux C,Lemaigre FP,Adili A,Yuan D,Weber A,Unger K,Heikenwälder M,Leclercq IA,Schwabe RF4. Journal of immunology (Baltimore, Md. : 1950)Mutagenesis of Ly49B reveals key structural elements required for promiscuous binding to MHC class I molecules and new insights into the molecular evolution of Ly49s.AuthorsMickiewicz KM,Gays F,Lewis RJ,Brooks CG5. PloS oneVisualization and identification of IL-7 producing cells in reporter mice.AuthorsMazzucchelli RI,Warming S,Lawrence SM,Ishii M,Abshari M,Washington AV,Feigenbaum L,Warner AC,Sims DJ,Li WQ,Hixon JA,Gray DH,Rich BE,Morrow M,Anver MR,Cherry J,Naf D,Sternberg LR,McVicar DW,Farr AG,Germain RN,Rogers K,Jenkins NA,Copeland NG,Durum SK

技术参数

产品应用 ICC;IF;IHC

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