F4/80 Monoclonal Antibody (BM8), APC

货号：17-4801-82,17-4801-80

规格：100μg,25μg

价格：2840,1029

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：BM8

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	2 µg/test(Flow）

Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor. It belongs to the EGF-TM7 family of proteins. As such it contains seven EGF-like domains on its extracellular N-terminus, seven transmembrane spanning sequences, and an intracellular C-terminal domain showing homology to other TM7 superfamily members. The F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal eosinophils, Langerhans cells, and some other dendritic cell subtypes, have been reported to express this antigen as well. Expression of F4/80 commences during early myeloid development in vivo and can be upregulated on BM cells stimulated in vitro with M-CSF. Some populations of macrophages, especially in the lymphoid microenvironment, may be devoid of F4/80.Applications Reported: BM8 has been reported for use in flow cytometric analysis.Applications Tested: The BM8 antibody has been tested by flow cytometric analysis of mouse resident peritoneal exudate cells. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationF4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

F4/80 Antibody (17-4801-82) in Flow Staining of C57Bl/6 resident peritoneal exudate cells with Anti-Mouse CD11b PE (Product # 12-0112-82) and 1 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 1 µg of Anti-Mouse F4/80 Antigen APC (right). Cells in the large scatter population were used for analysis.

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参考文献：

1. Oxidative medicine and cellular longevityGenetic Nrf2 Overactivation Inhibits the Deleterious Effects Induced by Hepatocyte-Specific c-met Deletion during the Progression of NASH."17-4801 was used in Flow cytometry/Cell sorting to elucidate the effect of Nrf2 overactivation on cellular events in c-met deficient hepatocytes."AuthorsRamadori P,Drescher H,Erschfeld S,Fragoulis A,Kensler TW,Wruck CJ,Cubero FJ,Trautwein C,Streetz KL,Kroy DC2. Immunology and cell biologyEvaluation of trained immunity by β-1, 3 (d)-glucan on murine monocytes in vitro and duration of response in vivo.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Scientific reportsExercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. The Journal of clinical investigationA CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Frontiers in immunologyserovar Typhimurium Infection.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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