F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 633
货号：A-21072
规格：250μl
价格：2807
产品类型：荧光二抗
品牌：Thermo Fisher
物种：兔
宿主：山羊
抗体亚型：IgG
荧光染料： Alexa Fluor 633
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Gamma Immunoglobins Heavy and Light chains | 用法： | 1-10 μg/mL（Flow）；4 μg/mL (ICC)；4 μg/mL (IF) |
Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据 |
| Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21072) in IFImmunofluorescence analysis of F (ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 633 was performed using HepG2 cells stained with alpha-1 antitrypsin Rabbit Polyclonal Primary Antibody (Product # PA5-16661). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Rabbit primary antibody (1:250 dilution)for 3 hours at room temperature. F (ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 633 (Product # A-21072) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha-1 antitrypsin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
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参考文献： |
| 1. Scientific reportsFSH protects mouse granulosa cells from oxidative damage by repressing mitophagy."A21072 was used in immunocytochemistry to show that follicle-stimulating hormone promotes granulosa cell survival by suppressing oxidative stress-induced mitophagy"AuthorsShen M,Jiang Y,Guan Z,Cao Y,Sun SC,Liu H2. DiabetesRetinal microglial activation and inflammation induced by amadori-glycated albumin in a rat model of diabetes."A-21072 was used in immunocytochemistry to elucidate the mechanisms by which diabetes activates microglia"AuthorsIbrahim AS,El-Remessy AB,Matragoon S,Zhang W,Patel Y,Khan S,Al-Gayyar MM,El-Shishtawy MM,Liou GI3. Molecular biology of the cellCritical roles for multiple formins during cardiac myofibril development and repair.AuthorsRosado M,Barber CF,Berciu C,Feldman S,Birren SJ,Nicastro D,Goode BL |
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