PCR Master Mix (2X)
货号：K0171,K0172
规格：200 reactions,1000 reactions
价格：201,780
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
| Thermo Scientific PCR Master Mix is a 2X concentrated solution ofTaqDNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.Note• The error rate ofTaqDNA Polymerase in PCR is 2.2 x 10-5errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs. |
优点： |
| -Convenient, ready-to-use mix-Thermostable—half life is more than 40 min at 95°C-Generates PCR products with 3'-dA overhangs-Incorporates modified nucleotides(e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides) |
组成成分： |
| ▪4 x 1.25 mL PCR Master Mix (2X), which includes:— Taq DNA polymerase (0.05 U/µL),— reaction buffer,— 4 mM MgCl2, and— 0.4 mM of each dNTP▪ 4 x 1.25 mL Nuclease-free waterStore at -20°C. |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling
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