Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 633/兔抗鼠 IgG (H+L)交叉吸附 荧光二抗, Alexa Fluor 633

货号：A-21063

规格：1 mg

价格：3091

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料： Alexa Fluor 633

点击查看所有Alexa Fluor荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1-10 μg/mL（Flow）；4 μg/mL (ICC)；1-10 μg/mL (IF)；1-10 μg/mL (IHC)

靶标信息 IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21063) in IFImmunofluorescence analysis of Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 633 (Product # A-21063) was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with mouse primary antibody (1:250 dilution) for 3 hours at room temperature. Rabbit anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 633 was used at concentration of 4µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Clinical science (London, England : 1979)Smad7 regulates compensatory hepatocyte proliferation in damaged mouse liver and positively relates to better clinical outcome in human hepatocellular carcinoma.2. The Journal of cell biologyCofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting.3. The international journal of biochemistry and cell biologyBeta-phenylethyl isothiocyanate mediated apoptosis; contribution of Bax and the mitochondrial death pathway.

技术参数

产品应用 ICC;IF;IHC;Flow

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