Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594/鸡抗兔 IgG (H+L)交叉吸附 荧光二抗, Alexa Fluor 594

货号：A-21442

规格：500μl

价格：3563

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：其它

抗体亚型：IgG

荧光染料：Alexa Fluor 594

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（IHC）；4 μg/mL (ICC)；4 μg/mL (IF)

This chicken anti-rabbit antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21442) in IFImmunofluorescence analysis of Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 was performed using HepG2 cells stained with alpha-1 antitrypsin Rabbit Polyclonal Primary Antibody (Product # PA5-16661). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Rabbit primary antibody (1:250 dilution) for 3 hours at room temperature. Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Product # A-21442) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha-1 antitrypsin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Neurochemical researchHemin protects against oxygen-glucose deprivation-induced apoptosis activation via neuroglobin in SH-SY5Y cells."A21442 was used in immunohistochemistry and western blot to explore the mechanism underlying the neuroprotective effect of hemin in oxygen-glucose deprivation-treated neurons"AuthorsWang YJ,Peng QY,Deng SY,Chen CX,Wu L,Huang L,Zhang LN2. International journal of molecular sciencesLeptin Receptor Metabolism Disorder in Primary Chondrocytes from Adolescent Idiopathic Scoliosis Girls."A21442 was used in western blot to elucidate the mechanisms that result in low metabolic activity of primary chondrocytes obtained from girls with adolescent idiopathic scoliosis"AuthorsWang YJ,Yu HG,Zhou ZH,Guo Q,Wang LJ,Zhang HQ3. Nature immunologyThe aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin."A21442 was used in immunohistochemistry - frozen section to suggest that air-pollution-induced atopic dermatitis occurs through activation of AhR"AuthorsHidaka T,Ogawa E,Kobayashi EH,Suzuki T,Funayama R,Nagashima T,Fujimura T,Aiba S,Nakayama K,Okuyama R,Yamamoto M4. Nature communicationsEngineering prokaryotic channels for control of mammalian tissue excitability."A21442 was used in immunohistochemistry to synthesize de novo excitable human tissues and augment impaired action potential conduction"AuthorsNguyen HX,Kirkton RD,Bursac N5. PLoS pathogensComparative Proteomics Reveals Strain-Specific β-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex."A21442 was used in immunocytochemistry to determine the rotavirus NSP1-host protein interaction network"AuthorsDing S,Mooney N,Li B,Kelly MR,Feng N,Loktev AV,Sen A,Patton JT,Jackson PK,Greenberg HB

技术参数

产品应用 ICC;IF;IHC

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