Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 594
货号：A27016
规格：1mg
价格：3319
产品类型：荧光二抗
品牌：Thermo Fisher
物种：山羊
宿主：兔
抗体亚型：IgG
荧光染料：Alexa Fluor 594
点击查看所有Alexa Fluor 荧光二抗
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Recombinant full-length protein | 用法： | 0.1-1 μg/mL (ICC)；0.1-1 μg/mL (IF) |
The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.Background/Target InformationSuperclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.
数据 |
| Goat IgG (H+L) Secondary Antibody (A27016) in IFSmooth muscle Actin (red) in U20S human osteocarcinoma cells was labeled with Goat anti-alpha-smooth muscle Actin primary antibody and visualized using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A27016) (0.4 µg/mL, 1:2500). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (Product # S36938) (1:50). To demonstrate multiplexing Apoptosis Inducing Factor (green) was labeled with anti-AIF mouse primary antibody (Product # 45-6200) and visualized with Rabbit anti-Mouse IgG (H+L), Alexa Fluor® 488 conjugate (Product # A-11059) (1:400). |
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| 二抗荧光二抗免疫组化一抗标签抗体 |
参考文献： |
| 1. Investigative ophthalmology and visual scienceMicroglia Activation and Recruitment of Circulating Macrophages During Ischemic Experimental Branch Retinal Vein Occlusion."A27016 was used in immunohistochemistry - paraffin section to examine histologic changes in Balb/c mice upon induction of experimental branch retinal vein occlusion"AuthorsEbneter A,Kokona D,Schneider N,Zinkernagel MS2. Experimental eye researchImaging of macrophage dynamics with optical coherence tomography in anterior ischemic optic neuropathy."A27016 was used in immunohistochemistry - paraffin section to propose that activated macrophages can be identified as hyperreflective dots in OCT"AuthorsKokona D,Häner NU,Ebneter A,Zinkernagel MS3. Investigative ophthalmology and visual scienceNoninvasive Quantification of Retinal Microglia Using Widefield Autofluorescence Imaging."A27016 was used in immunohistochemistry - paraffin section to confirm that widefield autofluorescence imaging can largely replace ex vivo quantification on retinal flat mounts"AuthorsKokona D,Schneider N,Giannakaki-Zimmermann H,Jovanovic J,Ebneter A,Zinkernagel M4. FEBS lettersBeta3 adrenergic receptor is involved in vascular injury in deoxycorticosterone acetate-salt hypertensive mice."A27016 was used in immunohistochemistry to elucidate the function and regulation mechanism of ADRB3 in the perivascular adipose tissue to determine how in contributes to hypertension"AuthorsSheng LJ,Ruan CC,Ma Y,Chen DR,Kong LR,Zhu DL,Gao PJ |
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