| Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21211) in IFImmunofluorescence analysis of Rabbit anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Rat primary antibody for 3 hours at room temperature. Rabbit anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate (Product # A-21211) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
| 1. Nature communicationsCovalent assembly of nanoparticles as a peptidase-degradable platform for molecular MRI."A21211 was used in flow cytometry to design ligand-targeted microparticles of iron oxide derived from multiple iron oxide nanoparticles coupled covalently through peptide linkers designed to be cleaved by intracellular macrophage proteases for detection of inflammation via magnetic r"AuthorsPerez-Balderas F,van Kasteren SI,Aljabali AA,Wals K,Serres S,Jefferson A,Sarmiento Soto M,Khrapitchev AA,Larkin JR,Bristow C,Lee SS,Bort G,De Simone F,Campbell SJ,Choudhury RP,Anthony DC,Sibson NR,Davis BG2. British journal of pharmacologyAntagonizing midkine accelerates fracture healing in mice by enhanced bone formation in the fracture callus."A-21211 was used in immunohistochemistry - paraffin section to discuss the effects of an Mdk antagonist on bone formation during fracture healing"AuthorsHaffner-Luntzer M,Heilmann A,Rapp AE,Roessler R,Schinke T,Amling M,Ignatius A,Liedert A3. The Journal of investigative dermatologyYap controls stem/progenitor cell proliferation in the mouse postnatal epidermis."A-21211 was used in immunohistochemistry - paraffin section to investigate genetic mechanisms that control stem and progenitor cell activation in the postnatal epidermis"AuthorsBeverdam A,Claxton C,Zhang X,James G,Harvey KF,Key B4. Journal of neuroimmunologyIntraperitoneal inoculation of Sandhoff mouse neonates with an HIV-1 based lentiviral vector exacerbates the attendant neuroinflammation and disease phenotype."A21211 was used in immunohistochemistry to assess the efficacy of VSV-G pseudotyped, defective HIV-1 based lentiviral vectors for the neonatal transfer of therapeutic genes following systemic administration in Sandhoff mouse pups"AuthorsKyrkanides S,Miller JN,Tallents RH,Brouxhon SM,Centola GM,Olschowka JA |
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