NxSeq® Single Indexing Kit, Set A
货号：15100-1
规格：12 x 4 rxn
价格：3009
产品类型：二代测序-建库
品牌：Lucigen
| The NxSeq® UltraLow DNA Library Kit and NxSeq® Single Indexing Kits allow you to build high quality DNA fragment libraries from extremely low DNA input amounts – as low as 50 pg depending on sample type and Illumina sequencer used. If you have more DNA, no problem; you can use as much as 75 ng of input DNA with this system. To generate these high quality libraries, we optimized each step of the protocol to ensure peak performance on Illumina sequencers. To help with the initial steps of DNA fragmentation, we’ve included guidance for mechanical shearing on an instrument like a Covaris LE220 and an optimized protocol for enzymatic fragmentation using dsDNA Shearase Plus from Zymo Research. Not only will you produce high quality libraries, but once you have your fragmented DNA, library prep is quick and easy - about 3 hours. This library and single indexing kits have been optimized for whole genome sequencing (WGS) and resequencing applications for de novo sequencing or SNV/mutation or copy number variation (CNV) identification. The system can, however, be used in other NGS applications such as ChIP-seq, exome-seq, and with other sample types such as metagenomic and FFPE DNA samples which require PCR-amplified DNA fragment libraries. |
| 优点： |
| ▪High Quality Data: High efficiency adaptor ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions；▪Sensitive:Construct DNA fragment libraries from as little as 50 pg to as much 75 ng of sheared/fragmented DNA；▪Minimal Bias: Robust, uniform PCR amplification improves coverage uniformity when working with low input amounts of genomic DNA |
| 数据： |
| Figure 1. Mechanics of NxSeq® UltraLow Library Construction Using Single Indexing Primers.This workflow figure illustrates how DNA fragment libraries are constructed using the NxSeq® UltraLow Library Prep Kit and NxSeq Single Indexing Kits. Note that some PCR fragments and products are not shown to simplify the illustration.Figure 2: Percentages of DNA fragments with correctly ligated adaptors measured by qPCR.Figure 3.Sequencing bias analysis for three different organisms with varying GC content |
| 相关产品： |
| ▪NxSeq® UltraLow DNA Library Kit, 12 Reactions（货号#15012-1）▪ NxSeq® Single Indexing Kit, Set B（货号#15200-1） |
技术参数 产品优点 - High Quality Data: High efficiency adaptor ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions
- Sensitive: Construct DNA fragment libraries from as little as 50 pg to as much 75 ng of sheared/fragmented DNA
- Minimal Bias: Robust, uniform PCR amplification improves coverage uniformity when working with low input amounts of genomic DNA
- Flexible:Extensively tested in de novo whole genome sequencing or resequencing, but compatible with other applications such as exome-seq, ChIP-seq and FFPE DNA samples.
- Fast: 3 hour protocol gets your samples on the sequencer quicker
- High Value: Cost-effective library and indexing kits which produce excellent sequencing results
产品应用 - Lowest input, highest efficiency, Illumina-compatible DNA fragment library prep kit
产品类型 建库试剂盒
组成成分 The NxSeq® UltraLow DNA Library Kit and NxSeq Single Indexing Kits are only compatible with Illumina sequencers.Each NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix for 4 library amplification reactions (48 total reaction for all primer sets). Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. Each NxSeq® Single Index equals the TruSeq® LT Index with the same number.
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