Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594/鸡抗大鼠IgG (H+L)交叉吸附荧光二抗, Alexa Fluor 594
货号：A-21471
规格：500μl
价格：3545
产品类型：荧光二抗
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：IgG
荧光染料：Alexa Fluor 594
点击查看所有Alexa Fluor 荧光二抗
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Gamma Immunoglobins Heavy and Light chains | 用法： | 2 μg/mL（Flow）；2 μg/mL (ICC)；1-10 μg/mL (IHC) |
Chicken anti-rat antibodies react with IgG heavy chains and all classes of immunoglobulin light chains from rat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.Background/Target InformationAnti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据 |
| Rat IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21471) in IFImmunofluorescence analysis of Chicken anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/mL Rat primary antibody for 3 hours at room temperature. Chicken anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor 594 conjugate (Product # A-21471) was used at a concentration of 2µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
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参考文献： |
| 1. Genes and cancerTargeting the PyMT Oncogene to Diverse Mammary Cell Populations Enhances Tumor Heterogeneity and Generates Rare Breast Cancer Subtypes.AuthorsSmith BA,Shelton DN,Kieffer C,Milash B,Usary J,Perou CM,Bernard PS,Welm BE2. Journal of the American Society of Nephrology : JASNCells derived from young bone marrow alleviate renal aging.AuthorsYang HC,Rossini M,Ma LJ,Zuo Y,Ma J,Fogo AB3. Proceedings of the National Academy of Sciences of the United States of AmericaAcid sphingomyelinase involvement in tumor necrosis factor alpha-regulated vascular and steroid disruption during luteolysis in vivo.AuthorsHenkes LE,Sullivan BT,Lynch MP,Kolesnick R,Arsenault D,Puder M,Davis JS,Rueda BR |
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