Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594/驴抗绵羊 IgG (H+L)交叉吸荧光二抗, Alexa Fluor 594

货号：A-11016

规格：500μl

价格：3625

产品类型：荧光二抗

品牌：Thermo Fisher

物种：其它

宿主：驴

抗体亚型：IgG

荧光染料：Alexa Fluor 594

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（Flow）；1-10 μg/mL (ICC)；1-10 μg/mL (IF)

Background/Target InformationAnti-Ovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11016) in IFSimultaneous detection of expression of five genes in a whole-mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes. Red: sog labeled using aminoallyl UTP (Product # A21663, A32765) and Alexa Fluor® 647 succinimidyl ester (Product # A-20006, A20106). Green: ind labeled with DNP, followed by rabbit anti-dinitrophenyl-KLH IgG antibody (Product # A-6430) prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (Product # Z-25305). Blue: en labeled with biotin and detected with HRP-streptavidin and Alexa Fluor® 405 tyramide (TSA™ Kit 39, Product # T30952). Yellow: wg labeled with digoxigenin and detected with sheep anti-digoxigenin IgG antibody and Alexa Fluor® 594 Donkey Anti-Sheep IgG antibody (Product # A-11016). Magenta: msh labeled with fluorescein and detected with mouse anti-fluorescein/Oregon Green® IgG2a antibody (Product # A-6421) and Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Product # A-11001, A11029). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.

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参考文献：

1. Neural plasticityActivation of Sphingosine 1-Phosphate Receptor 1 Enhances Hippocampus Neurogenesis in a Rat Model of Traumatic Brain Injury: An Involvement of MEK/Erk Signaling Pathway."A11016 was used in immunohistochemistry - paraffin section to examine changes in hippocampal S1PR1 expression after traumatic brain injury and the effects on neurogenesis and neurocognitive function"AuthorsYe Y,Zhao Z,Xu H,Zhang X,Su X,Yang Y,Yu X,He X2. Kidney internationalaccelerates glomerular disease."A11016 was used in immunohistochemistry - paraffin section to determine the function of thymosin in the kidneys"AuthorsVasilopoulou E,Kolatsi-Joannou M,Lindenmeyer MT,White KE,Robson MG,Cohen CD,Sebire NJ,Riley PR,Winyard PJ,Long DA3. PloS oneExpression of Prostatic Acid Phosphatase in Rat Circumvallate Papillae."A11016 was used in immunohistochemistry - free floating to examine prostatic acid phosphatase expression in rat taste buds"AuthorsNishida K,Kubota T,Matsumoto S,Kato J,Watanabe Y,Yamamoto A,Furui M,Ohishi A,Nagasawa K4. The Journal of comparative neurologyMelanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations."A11016 was used in immunohistochemistry to study melanopsin cells in primates"AuthorsLiao HW,Ren X,Peterson BB,Marshak DW,Yau KW,Gamlin PD,Dacey DM5. NeurologyHuman neurexin-3α antibodies associate with encephalitis and alter synapse development."A11016 was used in immunocytochemistry to characterize a novel autoimmune encephalitis"AuthorsGresa-Arribas N,Planagumà J,Petit-Pedrol M,Kawachi I,Katada S,Glaser CA,Simabukuro MM,Armangué T,Martínez-Hernández E,Graus F,Dalmau J

技术参数

产品应用 ICC;IF;Flow

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