Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568/驴抗羊 IgG (H+L) 交叉吸附荧光二抗, Alexa Fluor 568

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Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568/驴抗羊 IgG (H+L) 交叉吸附荧光二抗, Alexa Fluor 568

货号:A-11057

规格:500μl

价格:3589

产品类型:荧光二抗

品牌:Thermo Fisher

物种:山羊

宿主:驴

抗体亚型:IgG

荧光染料:Alexa Fluor 568

点击查看所有Alexa Fluor 荧光二抗

抗体类型:

荧光二抗

同型对照:

IgG

免疫原性:

Gamma Immunoglobins Heavy and Light chains

用法:

1-10 μg/mL(Flow);1-10 μg/mL (ICC);1-10 μg/mL (IF);1-10 μg/mL (IHC)

To minimize cross-reactivity, these donkey anti-goat IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against rabbit, rat, mouse, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 568 dye is a bright, orange/red-fluorescent dye with excitation ideally suited to the 568 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 568 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Background/Target InformationWe offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

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二抗荧光二抗免疫组化一抗标签抗体

参考文献:

1. eLifeTranscription factor Emx2 controls stereociliary bundle orientation of sensory hair cells."A11057 was used in immunohistochemistry to elucidate that Emx2 expression is required to reverse hair bundles from their 'default' polarity in the macula and neuromast"AuthorsJiang T,Kindt K,Wu DK2. Molecular systems biologyAdaptive resistance of melanoma cells to RAF inhibition via reversible induction of a slowly dividing de-differentiated state."A11057 was used in immunohistochemistry - paraffin section to find that exposure of tumor cells to RAF/MEK inhibitors elicits a heterogeneous response in which some cells die, some arrest, and the remainder adapt to drug"AuthorsFallahi-Sichani M,Becker V,Izar B,Baker GJ,Lin JR,Boswell SA,Shah P,Rotem A,Garraway LA,Sorger PK3. Gliareceptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases."A11057 was used in immunohistochemistry - paraffin section to study P2Y12 expression in human brain tissue"AuthorsMildner A,Huang H,Radke J,Stenzel W,Priller J4. BMC biologyAmyloid-like aggregation of provasopressin in diabetes insipidus and secretory granule sorting."A11057 was used in immunocytochemistry to discover two independent segments of provasopressin responsible for endoplasmic reticulum aggregation"AuthorsBeuret N,Hasler F,Prescianotto-Baschong C,Birk J,Rutishauser J,Spiess M5. Scientific reportsInvolvement of voltage-dependent anion channel (VDAC) in dengue infection."A11057 was used in immunocytochemistry to study the role of the voltage-dependent anion channel during dengue virus infection"AuthorsJitobaom K,Tongluan N,Smith DR
技术参数

产品应用 ICC;IF;IHC;Flow

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