| Rabbit IgG (H+L) Secondary Antibody (A27039) in IFImmunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 555 (Product # A27039) was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL of rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 555 (Product # A27039) was used at concentration of 0.5 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
| 1. Molecular visionmice."A27039 was used in immunohistochemistry - frozen section to evaluate the mechanisms of retinal degeneration"AuthorsDong E,Bachleda A,Xiong Y,Osawa S,Weiss ER2. eLifeInhibitor of ppGalNAc-T3-mediated O-glycosylation blocks cancer cell invasiveness and lowers FGF23 levels."A27039 was used in immunocytochemistry to attempt to identify a compound targeting the polypeptide N-acetylgalactosaminyltransferase 3 isozyme"AuthorsSong L,Linstedt AD3. Nature communicationsZyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules."A27039 was used in immunohistochemistry to evaluate the mechanisms of Weibel-Palade body exocytosis upon vascular injury"AuthorsHan X,Li P,Yang Z,Huang X,Wei G,Sun Y,Kang X,Hu X,Deng Q,Chen L,He A,Huo Y,Li D,Betzig E,Luo J4. The Journal of cell biologyRegulation of morphine-induced synaptic alterations: Role of oxidative stress, ER stress, and autophagy."A27039 was used in immunocytochemistry and immunohistochemistry to assess the contribution of platelet-derived growth factor in morphine-mediated synaptic alterations"AuthorsCai Y,Yang L,Hu G,Chen X,Niu F,Yuan L,Liu H,Xiong H,Arikkath J,Buch S5. Nature neuroscienceAge-related myelin degradation burdens the clearance function of microglia during aging."A27039 was used in immunohistochemistry to characterize age-related myelin fragmentation"AuthorsSafaiyan S,Kannaiyan N,Snaidero N,Brioschi S,Biber K,Yona S,Edinger AL,Jung S,Rossner MJ,Simons M |
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